产品目录

分子量：Approximately 21.0kDa,a single non-glycosylated polypeptide chain containing 180 amino acids.
储存条件：冷冻（-20℃）

概述

Murine FGF-18 is encoded by the FGF18 gene.By phylogenetic analysis and gene location analysis,FGF-18 is divided into FGF-8 subfamily which has three members FGF-8,FGF-17 and FGF-18.Using FGF knockout mice model,the numbers of this subfamily were testified that have crucial roles of in embryo development.FGF-18–/– mice have decreased expression of osteogenic markers and delayed long-bone ossification.FGF-18 has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells.In addition,it also has significant roles in lung development and has an anabolic effect on cartilage formation.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞95%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5ng/ml,corresponding to a specific activity of>2.0×106 IU/mg.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4,500mMNaCl.
内毒素	Less than 1 EU/μg of rMuFGF-18 as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQAELQK PFKYTTVTKR SRRIRPTHPG

相关搜索：重组小鼠FGF-18，FGF-18, Murine，Recombinant Murine Fibroblast Growth Factor-18